Anti-Mouse CD20/MS4A1 Recombinant Antibody (MB20-11) (MY257013)

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Overview

Catalog No.

MY257013

Description

Anti-Mouse CD20/MS4A1 Antibody (MB20-11) [MB20-11] (MY257013) is a mouse monoclonal antibody detecting Ms4a1 in ELISA, FACS, Functional assay, Research in vivo. Suitable for Mouse.

Highlights

●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse

Applications

ELISA, FACS, Functional assay, Research in vivo

Host species

Mouse

Isotype

IgG2c, kappa

Clone ID

MB20-11

Clonality

Monoclonal

Target

B-cell differentiation antigen Ly-44, B-lymphocyte antigen CD20, CD20, Cd20, Ly-44, Lymphocyte antigen 44, Membrane-spanning 4-domains subfamily A member 1, Ms4a1, Ms4a2

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P19437

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

B-lymphocyte antigen CD20 (MS4A1) is a ~31 kDa protein. B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR. CD20 is the therapeutic target of rituximab (MabThera) and obinutuzumab (Gazyva).

Note

For research use only

Images

Flow cytometry

Flow-cytometry using FITC anti-Mouse CD20 antibody. Mouse spleen cells were stained with an irrelevant antibody (Blue Histogram) or an FITC conjugated anti-Mouse CD20 monoclonal antibody (Catalog MY257013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing,cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using anti-Mouse CD20 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-Mouse CD20 monoclonal antibody (Catalog MY257013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience:MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Mouse CD20/MS4A1 Recombinant Antibody (MB20-11).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse CD20/MS4A1 Recombinant Antibody (MB20-11) (MY257013)

Overview

Images

References

Datasheet

SDS

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