Anti-Mouse CD248 Recombinant Antibody (G78) (MV681107)

Name: Anti-Mouse CD248 Recombinant Antibody (G78)
Brand: abinScience
SKU: MV681107
Price: 191 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

MV681107

Species reactivity

Human, Mouse

Applications

ELISA, FCM, in vivo study

Host species

Human

Isotype

IgG1, lambda

Clone ID

G78

Clonality

Monoclonal

Target

Endosialin, TEM1, CD164L1, Tumor endothelial marker 1, CD248

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q91V98

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Endosialin (CD248) is a ~81 kDa protein. Cell surface glycoprotein involved in various biological processes including angiogenesis, immune response modulation, and tissue remodeling and repair. Participates in pericyte proliferation through positive modulation of the PDGF receptor signaling pathway. Acts as a scaffold for factor X, triggering allosteric changes and the spatial re-alignment of factor X with the TF-factor VIIa complex, thereby enhancing coagulation activation. Modulates the insulin signaling pathway by interacting with insulin receptor/INSR and by diminishing its capacity to be autophosphorylated in response to insulin. Also regulates LPS-induced inflammatory responses in macrophages by favoring production of proinflammatory cytokines.

1. Opavsky, R. et al. (2001) The Journal of biological chemistry 276, 38795-807. PMID: 11489895

2. Naylor, AJ. et al. (2014) PloS one 9, e107146. PMID: 25243742

3. Benedet, PO. et al. (2024) EBioMedicine 99, 104906. PMID: 38061240

4. Hong, YK. et al. (2019) The Journal of investigative dermatology 139, 2204-2214.e7. PMID: 30986375

5. Kapopara, PR. et al. (2021) Journal of thrombosis and haemostasis : JTH 19, 1932-1947. PMID: 33830628

6. Cheng, TL. et al. (2021) Translational research : the journal of laboratory and clinical medicine 232, 150-162. PMID: 33737161

Note

For research use only.

Images

Flow Cytometry

Flow-cytometry using anti-mouse CD248 antibody.CD248 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD248 antibody monoclonal antibody (Catalog # MV681107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Mouse CD248 Antibody (G78)