Anti-Mouse CD28 Polyclonal Antibody (MY422014)

Name: Anti-Mouse CD28 Polyclonal Antibody
Brand: abinScience
SKU: MY422014
Price: 168 USD
Availability: InStock

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Price(USD): $

Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

MY422014

Species reactivity

Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse CD28 (Lys21-Lys137).

Target

T-cell-specific surface glycoprotein CD28 (CD antigen CD28)

Purification

Purified by antigen affinity column.

Accession

P31041

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

T-cell-specific surface glycoprotein CD28 is a ~25 kDa protein. Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis. Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells. Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets. Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways. In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells.

1. Shahinian, A. et al. (1993) Science (New York, N.Y.) 261, 609-12. PMID: 7688139

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Mouse CD28 polyclonal antibody at 1ug/mL on recombinant Mouse CD28 (Catalog No. MY422012).

References

Datasheet