Anti-Mouse CD321/F11R Polyclonal Antibody (MV592014)

Name: Anti-Mouse CD321/F11R Polyclonal Antibody
Brand: abinScience
SKU: MV592014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

MV592014

Species reactivity

Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse CD321/F11R (Gly53-Glu233).

Target

Junctional adhesion molecule A (JAM-A) (Junctional adhesion molecule 1) (JAM-1) (CD antigen CD321)

Purification

Purified by antigen affinity column.

Accession

O88792

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Junctional adhesion molecule A (CD321/F11R) is a ~32 kDa protein. Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration.

1. Ebnet, K. et al. (2001) The EMBO journal 20, 3738-48. PMID: 11447115

2. Martìn-Padura, I. et al. (1998) The Journal of cell biology 142, 117-27. PMID: 9660867

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-F11R polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse lung lysate
Lane 2: Rat lung lysate

References

Datasheet

Anti-Mouse CD321/F11R Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse CD321/F11R Polyclonal Antibody (MV592014)

Overview

Images

References

Datasheet

SDS

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