Anti-Mouse CD33 Polyclonal Antibody (MB185014)

Name: Anti-Mouse CD33 Polyclonal Antibody
Brand: abinScience
SKU: MB185014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

MB185014

Description

Anti-Mouse CD33 Polyclonal Antibody (MB185014) is a rabbit polyclonal antibody detecting CD33 in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse CD33 (Ala12-Val231).

Target

Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, CD33, gp67, SIGLEC3

Purification

Purified by antigen affinity column.

Accession

Q63994

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Myeloid cell surface antigen CD33 is a ~44 kDa protein. Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially binds sialic acid to the short O-linked glycans of certain mucins. CD33 is the therapeutic target of gemtuzumab ozogamicin (Mylotarg).

1. Brinkman-Van der Linden, EC. et al. (2003) Molecular and cellular biology 23, 4199-206. PMID: 12773563

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Mouse CD33 polyclonal antibody at 1ug/mL on recombinant Mouse CD33 (Catalog No. MB185012).

References

Datasheet

Anti-Mouse CD33 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse CD33 Polyclonal Antibody (MB185014)

Overview

Images

References

Datasheet

SDS

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