Anti-Mouse CD55/DAF Polyclonal Antibody (MY307014)

Name: Anti-Mouse CD55/DAF Polyclonal Antibody
Brand: abinScience
SKU: MY307014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

MY307014

Description

Anti-Mouse CD55 Polyclonal Antibody (MY307014) is a rabbit polyclonal antibody detecting CD55 in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse CD55 (Asn44-Lys289).

Target

Complement decay-accelerating factor, CR, DAF, CD55

Purification

Purified by antigen affinity column.

Accession

Q61475

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Complement decay-accelerating factor, GPI-anchored (CD55) is a ~42 kDa protein. This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD55 polyclonal antibody at 1ug/mL on various samples.
Lane 1: 293T cell lysate

References

Datasheet

Anti-Mouse CD55/DAF Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse CD55/DAF Polyclonal Antibody (MY307014)

Overview

Images

References

Datasheet

SDS

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