Anti-Mouse CD86/B7-2 Recombinant Antibody (GL1), PE (MW776127)

Datasheet Share product Copy info

Price(USD): $

Spec:

50T
100T

Number:

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	MW776127
Description	Anti-Mouse CD86/B7-2 Antibody (GL1), PE [GL1] (MW776127) is a rat monoclonal antibody detecting CD86 in FCM. Suitable for Mouse. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Mouse
Applications	FCM
Host species	Rat
Isotype	IgG2a, kappa
Clone ID	GL1
Conjugation	PE
Clonality	Monoclonal
Target	BU63, B70, CD28LG2, CTLA-4 counter-receptor B7.2, Activation B7-2 antigen, T-lymphocyte activation antigen CD86, CD86, FUN-1
Endotoxin level	Please contact with the lab for this information.
Accession	P42082
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	T-lymphocyte activation antigen CD86 (B7-2) is a ~34 kDa protein. Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation. 1. Lucas, CR. et al. (2013) Journal of immunology (Baltimore, Md. : 1950) 190, 723-36. PMID: 23241883 2. Freeman, GJ. et al. (1993) The Journal of experimental medicine 178, 2185-92. PMID: 7504059 3. Chen, C. et al. (1994) Journal of immunology (Baltimore, Md. : 1950) 152, 4929-36. PMID: 7513726
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

References

Datasheet

Anti-Mouse CD86/B7-2 Recombinant Antibody (GL1), PE.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse CD86/B7-2 Recombinant Antibody (GL1), PE (MW776127)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us