Anti-Mouse CD8B//Lyt3 Recombinant Antibody (YTS156.7.7), APC (MY433137)

Name: Anti-Mouse CD8B//Lyt3 Recombinant Antibody (YTS156.7.7), APC
Brand: abinScience
SKU: MY433137
Price: 256 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.	MY433137
Species reactivity	Mouse
Applications	FCM
Host species	Rat
Isotype	IgG2b, kappa
Clone ID	YTS156.7.7
Conjugation	APC
Clonality	Monoclonal
Target	T-cell surface glycoprotein CD8 beta chain, Lymphocyte antigen 3, T-cell membrane glycoprotein Ly-3, T-cell surface glycoprotein Lyt-3, CD8b, Cd8b1, Ly-3, Lyt-3, Lyt3, cd8
Endotoxin level	Please contact with the lab for this information.
Accession	P10300
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	T-cell surface glycoprotein CD8 beta chain (CD8b/Lyt3) is a ~24 kDa protein. Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex.
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

Flow Cytometry

Flow-cytometry using APC anti-Mouse CD8b antibody. Mouse spleen cells were stained with an irrelevant antibody (Green Histogram) or an APC anti-Mouse CD8b antibody monoclonal antibody (Catalog # MY433137 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet