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Anti-Mouse CRH Polyclonal Antibody (MY109014)

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Overview
Catalog No.MY109014
Description
Anti-Mouse CRH Polyclonal Antibody (MY109014) is a rabbit polyclonal antibody detecting Crh in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse CRH (Ser145-Lys187).
Target Corticoliberin, Corticotropin-releasing factor, CRF, Corticotropin-releasing hormone, Crh, Gm1347
Purification Purified by antigen affinity column.
Accession Q8CIT0
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Corticoliberin (CRH) is a ~20 kDa protein. Hormone regulating the release of corticotropin from pituitary gland. Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile.

1. Sun, Y. et al. (2013) Gastroenterology 144, 1478-87, 1487.e1-8. PMID: 23470617
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Mouse CRH Polyclonal Antibody

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