Anti-Mouse CXCL12/SDF-1 Recombinant Antibody (30D8) (MW376013)

Name: Anti-Mouse CXCL12/SDF-1 Recombinant Antibody (30D8)
Brand: abinScience
SKU: MW376013
Price: 110 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

MW376013

Species reactivity

Human, Mouse

Applications

ELISA, FCM

Host species

Mouse

Isotype

IgG1, kappa

Clone ID

30D8

Clonality

Monoclonal

Target

Intercrine reduced in hepatomas, hSDF-1, Stromal cell-derived factor 1, SDF-1, Pre-B cell growth-stimulating factor, SDF1, SDF1A, C-X-C motif chemokine 12, SDF1B, CXCL12, IRH, PBSF, hIRH

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P40224

RRID

Anti-Mouse CXCL12/SDF-1 Antibody (30D8) (abinScience Cat# MW376013, RRID:AB_3725651)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Stromal cell-derived factor 1 (CXCL12/SDF-1) is a ~10 kDa protein. Chemoattractant active on T-lymphocytes and monocytes but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for CXCL12/SDF-1. Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 in a CXCR4-independent manner. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase.

1. Nagasawa, T. et al. (1996) Nature 382, 635-8. PMID: 8757135

2. Nagasawa, T. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 2305-9. PMID: 8134392

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Mouse CXCL12/SDF-1 Antibody (30D8).
Western blot

Western blot analysis was performed using anti-human CXCL12/SDF-1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CXCL12/SDF-1 (Catalog No: HW376012)
Lane 2: negative control

References

Datasheet

Anti-Mouse CXCL12/SDF-1 Recombinant Antibody (30D8).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse CXCL12/SDF-1 Recombinant Antibody (30D8) (MW376013)

Overview

Images

References

Datasheet

SDS

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