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Anti-Mouse CYP1A1 Polyclonal Antibody (MY456014)

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Overview
Catalog No.MY456014
Description
Anti-Mouse CYP1A1 Polyclonal Antibody (MY456014) is a rabbit polyclonal antibody detecting CYP1A1. Suitable for Rat and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityMouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse CYP1A1 (Phe52-Ala524).
Target CYP1A1, CYPIA1, Cytochrome P450 1A1, Cytochrome P450 form 6, Cytochrome P450-C, Cytochrome P450-P1, EC:1.14.14.1, EC:4.2.1.152, Hydroperoxy icosatetraenoate dehydratase
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P00184
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Cytochrome P450 1A1 (CYP1A1) is a ~59 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15alpha and C16alpha positions. Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation.

Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Mouse CYP1A1 Polyclonal Antibody

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