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Anti-Mouse DLL4 Polyclonal Antibody (MV571014)

Anti-Mouse DLL4 Polyclonal Antibody
Anti-Mouse DLL4 Polyclonal Antibody
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Overview
Catalog No.MV571014
Description
Anti-Mouse DLL4 Polyclonal Antibody (MV571014) is a rabbit polyclonal antibody detecting DLL4 in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse DLL4 (Gly27-Ser177).
Target Delta-like protein 4, Drosophila Delta homolog 4, Delta4, DLL4
Purification Purified by antigen affinity column.
Accession Q9JI71
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Delta-like protein 4 (DLL4) is a ~74 kDa protein. Involved in the Notch signaling pathway as Notch ligand. Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types.

1. Yoneya, T. et al. (2001) Journal of biochemistry 129, 27-34. PMID: 11134954
2. Luo, H. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, E553-62. PMID: 22323600
Note For research use only.
Images
  • Anti-Mouse DLL4 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-DLL4 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Rat lung lysate
    Lane 2: Rat kidney lysate
    Lane 3: Rat pancears lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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