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Anti-Mouse-ear cress CENH3 Polyclonal Antibody (PR109014)

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Overview
Catalog No.PR109014
Description
Anti-Mouse-ear cress CENH3 Polyclonal Antibody (PR109014) is a rabbit polyclonal antibody detecting CENH3 in ELISA, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
Species reactivityArabidopsis thaliana (Mouse-ear cress)
ApplicationsELISA, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse-ear cress CENH3 (Gly83-Trp178).
Target Histone H3-like centromeric protein CENH3, Centromeric histone CENH3, Centromeric histone H3, Histone H3-like centromeric protein HTR12, CENH3, HTR12, At1g01370, F6F3.17
Purification Purified by antigen affinity column.
Accession Q8RVQ9
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Histone H3-like centromeric protein CENH3 is a ~19 kDa protein. Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.

Note For research use only.
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Formula
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V₁ (Stock Vol.)
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C₂ (Working Conc.)
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Anti-Mouse-ear cress CENH3 Polyclonal Antibody

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