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Anti-Mouse EGFLAM Polyclonal Antibody (MP294014)

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Overview
Catalog No.MP294014
Species reactivityMouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse EGFLAM (Asp40-Thr236).
Target Pikachurin (EGF-like, fibronectin type-III and laminin G-like domain-containing protein) (Nectican)
Purification Purified by antigen affinity column.
Accession Q4VBE4
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Pikachurin (EGFLAM) is a ~110 kDa protein. Involved in both the retinal photoreceptor ribbon synapse formation and physiological functions of visual perception. Plays a key role in the synaptic organization of photoreceptors by mediating transsynaptic interaction between alpha-dystroglycan and GPR179 on the postsynaptic membrane. Necessary for proper bipolar dendritic tip apposition to the photoreceptor ribbon synapse. Promotes matrix assembly and cell adhesiveness.

1. Sato, S. et al. (2008) Nature neuroscience 11, 923-31. PMID: 18641643
2. Manabe, R. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 12849-54. PMID: 18757743
3. Orlandi, C. et al. (2018) Cell reports 25, 130-145.e5. PMID: 30282023
Note For research use only.
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