Anti-Mouse EGFR/ERBB1 Recombinant Antibody (SAA2220), PE (MF004137)

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Catalog No.	MF004137
Description	Anti-Mouse EGFR/ERBB1 Antibody (SAA2220), PE [SAA2220] (MF004137) is a rabbit monoclonal antibody detecting ERBB1 in ELISA, FCM, WB. Highlights ●Rabbit Monoclonal — High specificity, affinity, and excellent lot-to-lot consistency. ●Affinity Purified — Minimal background and high purity for reliable results. ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
Applications	ELISA, FCM, WB
Host species	Rabbit
Isotype	IgG
Clone ID	SAA2220
Conjugation	PE
Clonality	Monoclonal
Target	HER1, Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGFR, ERBB1, ERBB,
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Affinity chromatography.
Accession	Q01279
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	Epidermal growth factor receptor (EGFR/ERBB1) is a ~134 kDa protein. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. EGFR is the therapeutic target of cetuximab (Erbitux) and panitumumab (Vectibix). 1. Fazioli, F. et al. (1993) The EMBO journal 12, 3799-808. PMID: 8404850 2. Peng, SC. et al. (2010) Nucleic acids research 38, 7446-57. PMID: 20639532 3. Eagles, DA. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119. PMID: 35131940
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Anti-Mouse EGFR/ERBB1 Recombinant Antibody (SAA2220), PE.pdf

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Anti-Mouse EGFR/ERBB1 Recombinant Antibody (SAA2220), PE (MF004137)

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