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Anti-Mouse FADD Polyclonal Antibody (MC591014)

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Anti-Mouse FADD Polyclonal Antibody
Anti-Mouse FADD Polyclonal Antibody
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Overview
Catalog No.MC591014
Description
Anti-Mouse FADD Polyclonal Antibody (MC591014) is a rabbit polyclonal antibody detecting Fadd in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse FADD (Met1-Pro205).
Target FAS-associated death domain protein, FAS-associating death domain-containing protein, Mediator of receptor induced toxicity, Fadd, Mort1
Purification Purified by antigen affinity column.
Accession Q61160
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

FAS-associated death domain protein (FADD) is a ~22 kDa protein. Apoptotic adapter molecule that recruits caspases CASP8 or CASP10 to the activated FAS/CD95 or TNFRSF1A/TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. Active CASP8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling.

Note For research use only.
Images
  • Anti-Mouse FADD Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-FADD polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: A549 cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: Jurkat cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Mouse FADD Polyclonal Antibody

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