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Anti-Mouse FZD5 Polyclonal Antibody (MC526014)

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Overview
Catalog No.MC526014
Description
Anti-Mouse FZD5 Polyclonal Antibody (MC526014) is a rabbit polyclonal antibody detecting FZD5 in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse FZD5 (Thr37-Ala126).
Target Frizzled-5, FzE5, C2orf31, hFz5, FZD5, Fz-5
Purification Purified by antigen affinity column.
Accession Q9EQD0
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Frizzled-5 (FZD5) is a ~64 kDa protein. Receptor for Wnt proteins. Functions in the canonical Wnt/beta-catenin signaling pathway. In vitro activates WNT2, WNT10B, WNT5A, but not WNT2B or WNT4 signaling. In neurons, activation by WNT7A promotes formation of synapses. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable).

1. Ishikawa, T. et al. (2001) Development (Cambridge, England) 128, 25-33. PMID: 11092808
2. Carmon, KS. et al. (2008) Biochemical and biophysical research communications 368, 285-91. PMID: 18230341
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Mouse FZD5 Polyclonal Antibody

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