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Anti-Mouse GGT5 Polyclonal Antibody (MW691014)

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Overview
Catalog No.MW691014
Description
Anti-Mouse GGT5 Polyclonal Antibody (MW691014) is a rabbit polyclonal antibody detecting Ggt5 in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse GGT5 (Ser30-Tyr573).
Target Glutathione hydrolase 5 proenzyme, 3.4.19.13, Gamma-glutamyl leukotrienase, GGL, Gamma-glutamyltransferase 5, GGT 5, 2.3.2.2, Gamma-glutamyltransferase-like activity 1, Gamma-glutamyltranspeptidase 5, Leukotriene-C4 hydrolase, 3.4.19.14, Glutathione hydrolase 5 heavy chain, Glutathione hydrolase 5 light chain, Ggt5, Ggtla1
Purification Purified by antigen affinity column.
Accession Q9Z2A9
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Glutathione hydrolase 5 proenzyme (GGT5) is a ~61 kDa protein. Cleaves the gamma-glutamyl bond of extracellular glutathione tripeptide (gamma-Glu-Cys-Gly) and certain glutathione conjugates. Hydrolyzes glutathione releasing L-Glu and Cys-Gly dipeptide which is further metabolized to maintain extracellular cysteine levels but also to provide cysteine necessary for intracellular glutathione synthesis. Among glutathione-S-conjugates metabolizes leukotriene C4 (LTC4) and S-geranylgeranyl-glutathione (GGG), but is inactive toward gamma-glutamyl leucine. Converts extracellular LTC4 to LTD4 during acute inflammatory response. Acts as a negative regulator of GGG bioactivity.

1. Shi, ZZ. et al. (2001) Molecular and cellular biology 21, 5389-95. PMID: 11463821
2. Han, B. et al. (2002) The American journal of pathology 161, 481-90. PMID: 12163373
3. Lu, E. et al. (2019) Nature 567, 244-248. PMID: 30842656
4. Carter, BZ. et al. (1998) The Journal of biological chemistry 273, 28277-85. PMID: 9774450
5. Gallman, AE. et al. (2021) Science immunology 6. PMID: 34088745
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-Mouse GGT5 Polyclonal Antibody

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