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Anti-Mouse GZMA/Granzyme A Polyclonal Antibody (MY375014)

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Overview
Catalog No.MY375014
Description
Anti-Mouse GZMA/Granzyme A Polyclonal Antibody (MY375014) is a rabbit polyclonal antibody detecting Granzyme A in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse GZMA/Granzyme A (Val36-Val260).
Target Granzyme A, 3.4.21.78, Autocrine thymic lymphoma granzyme-like serine protease, CTLA-3, Fragmentin-1, T cell-specific serine protease 1, TSP-1, Gzma, Ctla-3, Ctla3, Mtsp-1
Purification Purified by antigen affinity column.
Accession P11032
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Granzyme A (GZMA) is a ~28 kDa protein. Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA.

1. Masson, D. et al. (1987) Cell 49, 679-85. PMID: 3555842
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Mouse GZMA/Granzyme A Polyclonal Antibody

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