Anti-Mouse HMGB1 Recombinant Antibody (DPH1.1) (MY200013)

Name: Anti-Mouse HMGB1 Recombinant Antibody (DPH1.1)
Brand: abinScience
SKU: MY200013
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

MY200013

Species reactivity

Human, Mouse, Rat, Bovine, Dog, Horse, Pig

Applications

FCM, FuncS, IF, WB

Host species

Rat

Isotype

IgG1, kappa

Clone ID

DPH1.1

Clonality

Monoclonal

Target

High mobility group protein B1, HMGB1, HMG1, HMG-1, High mobility group protein 1

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P63158

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

High mobility group protein B1 (HMGB1) is a ~24 kDa protein. Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as a sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy.

1. Yang, H. et al. (2013) Journal of leukocyte biology 93, 865-73. PMID: 23446148

2. Li, G. et al. (2013) Frontiers in immunology 4, 68. PMID: 23519706

3. Li, G. et al. (2013) Seminars in cancer biology 23, 380-90. PMID: 23994764

4. Lee, SA. et al. (2014) Yonsei medical journal 55, 1165-76. PMID: 25048472

5. Mitola, S. et al. (2006) Journal of immunology (Baltimore, Md. : 1950) 176, 12-5. PMID: 16365390

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Mouse HMGB1 Antibody (DPH1.1)
Western blot

Western blot analysis was performed using anti-HMGB1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human HMGB1 (Catalog No: HY200011)
Lane 2: negative control

References

Datasheet

Anti-Mouse HMGB1 Recombinant Antibody (DPH1.1).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse HMGB1 Recombinant Antibody (DPH1.1) (MY200013)

Overview

Images

References

Datasheet

SDS

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