Anti-Mouse HSPB1/HSP27 Polyclonal Antibody (MY322014)

Name: Anti-Mouse HSPB1/HSP27 Polyclonal Antibody
Brand: abinScience
SKU: MY322014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

MY322014

Description

Anti-Mouse HSPB1/HSP27 Polyclonal Antibody (MY322014) is a rabbit polyclonal antibody detecting HspB1 in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse HSPB1/HSP27 (Ala76-Pro174).

Target

Heat shock protein beta-1, HspB1, Growth-related 25 kDa protein, Heat shock 25 kDa protein, HSP 25, Heat shock 27 kDa protein, HSP 27, p25, Hspb1, Hsp25, Hsp27

Purification

Purified by antigen affinity column.

Accession

P14602

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Heat shock protein beta-1 (HSPB1/HSP27) is a ~23 kDa protein. Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins.

1. Huang, L. et al. (2007) Genesis (New York, N.Y. : 2000) 45, 487-501. PMID: 17661394

Note

For research use only.

Images

Western blot

Lane 1: MCF-7 cell lysate
Lane 2: Mouse heart lysate
Lane 3: Rat heart lysate
Lane 4: Mouse skeletal muscle lysate

References

Datasheet

Anti-Mouse HSPB1/HSP27 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse HSPB1/HSP27 Polyclonal Antibody (MY322014)

Overview

Images

References

Datasheet

SDS

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