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Anti-Mouse IgE/IGHE Recombinant Antibody (84.1C) (MV064013)

Anti-Mouse IgE/IGHE Recombinant Antibody (84.1C)
Anti-Mouse IgE/IGHE Recombinant Antibody (84.1C)
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Overview
Catalog No.MV064013
Description
Anti-Mouse IgE/IGHE Recombinant Antibody (84.1C) (MV064013) is a rat monoclonal antibody detecting IgE/IGHE in ELISA. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
Species reactivityMouse
ApplicationsELISA
Host speciesRat
ClonalityMonoclonal
IsotypeIgG1, kappa
Clone ID84.1C
Target IGHE, Ig epsilon chain C region, Ig epsilon chain C region ND, Immunoglobulin heavy constant epsilon
Endotoxin level < 10 EU/mg
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P06336
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Immunoglobulin heavy constant epsilon (IgE/IGHE) is a ~59 kDa protein. Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain.

1. Schroeder, HW. et al. (2010) The Journal of allergy and clinical immunology 125, S41-52. PMID: 20176268
2. McHeyzer-Williams, M. et al. (2011) Nature reviews. Immunology 12, 24-34. PMID: 22158414
3. Teng, G. et al. (2007) Annual review of genetics 41, 107-20. PMID: 17576170
Note For research use only.
Isotype Control Rat IgG1, kappa Recombinant Isotype Control Antibody (RTK2071) (RV080407)
Images
  • Anti-Mouse IgE/IGHE Recombinant Antibody (84.1C)

    SDS-PAGE

    SDS-PAGE for Anti-Mouse IgE/IGHE Antibody (84.1C).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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