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Anti-Mouse IL-21 Polyclonal Antibody (MV658014)

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Overview
Catalog No.MV658014
Description
Anti-Mouse IL21 Polyclonal Antibody (MV658014) is a rabbit polyclonal antibody detecting IL21 in ELISA, IHC, WB. Suitable for Rattus norvegicus and Mus musculus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse IL21 (Pro25-Ser146).
Target IL21
Purification Purified by antigen affinity column.
Accession Q9ES17
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesInterleukin-21, IL-21, Il21
Background

Interleukin-21 (IL21) is a ~16 kDa protein. Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection.

1. Pène, J. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 172, 5154-7. PMID: 15100251
2. Karnowski, A. et al. (2012) The Journal of experimental medicine 209, 2049-64. PMID: 23045607
3. Kasaian, MT. et al. (2002) Immunity 16, 559-69. PMID: 11970879
4. Brandt, K. et al. (2003) Blood 102, 4090-8. PMID: 12893770
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Mouse IL-21 Polyclonal Antibody

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