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Anti-Mouse IL31 Polyclonal Antibody (MV678014)

Anti-Mouse IL31 Polyclonal Antibody
Anti-Mouse IL31 Polyclonal Antibody
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Overview
Catalog No.MV678014
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse IL31 (Ala31-Cys163).
Tested applications WB
Target Interleukin-31 (IL-31)
Purification Purified by antigen affinity column.
Accession Q6EAL8
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Interleukin-31 (IL31) is a ~18 kDa protein. Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. May function in skin immunity. Enhances myeloid progenitor cell survival in vitro. Induces RETNLA and serum amyloid A protein expression in macrophages.

1. Dillon, SR. et al. (2004) Nature immunology 5, 752-60. PMID: 15184896
2. Broxmeyer, HE. et al. (2007) Experimental hematology 35, 78-86. PMID: 17379091
3. Edukulla, R. et al. (2015) The Journal of biological chemistry 290, 13510-20. PMID: 25847241
Note For research use only.
Images
  • Anti-Mouse IL31 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-IL31 polyclonal antibody at 1µg/ml on various samples.
    Lane 1: RAW264.7 cell lysate
    Lane 2: Mouse lung lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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