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Anti-Mouse IL36G/IL-1F9 Polyclonal Antibody (MV952014)

Anti-Mouse IL36G/IL-1F9 Polyclonal Antibody
Anti-Mouse IL36G/IL-1F9 Polyclonal Antibody
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Overview
Catalog No.MV952014
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse IL36G/IL-1F9 (Thr16-Ser164).
Target Interleukin-36 gamma (Interleukin-1 family member 9) (IL-1F9)
Purification Purified by antigen affinity column.
Accession Q8R460
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Interleukin-36 gamma (IL36G/IL-1F9) is a ~18 kDa protein. Functions as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2/IL1RL2. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. May play a role in pro-inflammatory responses during particular neutrophilic airway inflammation. May be involved in the innate immune response to fungal pathogens.

Note For research use only.
Images
  • Anti-Mouse IL36G/IL-1F9 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-IL36G polyclonal antibody at 1µg/ml on various samples.
    Lane 1: Mouse spleen lysate
    Lane 2: Mouse liver lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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