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Anti-Mouse IL-5 Polyclonal Antibody (MY218014)

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Overview
Catalog No.MY218014
Description
Anti-Mouse IL5 Polyclonal Antibody (MY218014) is a rabbit polyclonal antibody detecting Il5. Suitable for Rat and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityMouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse IL5 (Met21-Gly133).
Target B-cell growth factor II, BCGF-II, Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, IL-5, Il-5, Il5, Interleukin-5, T-cell replacing factor, TRF
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P04401
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Interleukin-5 (IL5) is a ~15 kDa protein. Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation. Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively. IL5 is the therapeutic target of mepolizumab (Nucala).

1. Vallance, BA. et al. (1999) The American journal of physiology 277, G400-8. PMID: 10444455
2. Barry, SC. et al. (1991) Cytokine 3, 339-44. PMID: 1873482
3. Karasuyama, H. et al. (1988) The Journal of experimental medicine 167, 1377-90. PMID: 3128631
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Mouse IL-5 Polyclonal Antibody

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