Anti-Mouse LGALS3/Galectin-3 Polyclonal Antibody (MB167014)

Name: Anti-Mouse LGALS3/Galectin-3 Polyclonal Antibody
Brand: abinScience
SKU: MB167014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

MB167014

Species reactivity

Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse LGALS3/Galectin-3 (Met1-Ile264).

Target

Galectin-3 (Gal-3) (35 kDa lectin) (Carbohydrate-binding protein 35) (CBP 35) (Galactose-specific lectin 3) (IgE-binding protein) (L-34 galactoside-binding lectin) (Laminin-binding protein) (Lectin L-29) (Mac-2 antigen)

Purification

Purified by antigen affinity column.

Accession

P16110

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Galectin-3 (Gal-3) is a ~27 kDa protein. Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

1. Fukushi, J. et al. (2004) Molecular biology of the cell 15, 3580-90. PMID: 15181153

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-LGALS3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: MCF-7 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Caco2 cell lysate

References

Datasheet

Anti-Mouse LGALS3/Galectin-3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse LGALS3/Galectin-3 Polyclonal Antibody (MB167014)

Overview

Images

References

Datasheet

SDS

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