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Anti-Mouse LHFPL5 Polyclonal Antibody (MC068014)

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Overview
Catalog No.MC068014
Description
Anti-Mouse LHFPL5 Polyclonal Antibody (MC068014) is a rabbit polyclonal antibody detecting LHFPL tetraspan subfamily member 5 protein isoform X2; Lhfpl5 in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant mouse LHFPL5 (Ile50-Met98&Asp150-Arg176&Arg202-Val219).
Target LHFPL tetraspan subfamily member 5 protein isoform X2; Lhfpl5
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q4KL25
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

LHFPL tetraspan subfamily member 5 protein (LHFPL5) is a ~24 kDa protein. Auxiliary subunit of the mechanotransducer (MET) non-specific cation channel complex located at the tips of the shorter stereocilia of cochlear hair cells and that mediates sensory transduction in the auditory system. The MET complex is composed of two dimeric pore-forming ion-conducting transmembrane TMC (TMC1 or TMC2) subunits, and aided by several auxiliary proteins including LHFPL5, TMIE, CIB2/3 and TOMT, and the tip-link PCDH15. Functionally couples PCDH15 to the transduction channel.

1. Longo-Guess, CM. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 7894-9. PMID: 15905332
2. Xiong, W. et al. (2012) Cell 151, 1283-95. PMID: 23217710
3. Fettiplace, R. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119, e2210849119. PMID: 36191207
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Mouse LHFPL5 Polyclonal Antibody

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