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Anti-Mouse MADCAM1 Polyclonal Antibody (MC589014)

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Overview
Catalog No.MC589014
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse MADCAM1 (Gln22-Ser222).
Target Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) (mMAdCAM-1)
Purification Purified by antigen affinity column.
Accession Q61826
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Mucosal addressin cell adhesion molecule 1 (MADCAM1) is a ~43 kDa protein. Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both the integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Both isoform 1 and isoform 2 can adhere to integrin alpha-4/beta-7. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.

Note For research use only.
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Formula
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V₁ (Stock Vol.)
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C₂ (Working Conc.)
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Anti-Mouse MADCAM1 Polyclonal Antibody

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