Anti-Mouse MAPT/Tau/PHF-tau Recombinant Antibody (TAU-5) (MY086013)

Name: Anti-Mouse MAPT/Tau/PHF-tau Recombinant Antibody (TAU-5)
Brand: abinScience
SKU: MY086013
Price: 126 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

MY086013

Species reactivity

Bovine, Human

Applications

IF, IHC-P, IP, WB

Host species

Mouse

Isotype

IgG1, kappa

Clone ID

TAU-5

Clonality

Monoclonal

Target

MAPTL, Paired helical filament-tau, MTBT1, Microtubule-associated protein tau, TAU, Neurofibrillary tangle protein, MAPT, PHF-tau

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P10637

RRID

Anti-Mouse MAPT/Tau/PHF-tau Antibody (TAU-5) (abinScience Cat# MY086013, RRID:AB_3726999)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Microtubule-associated protein tau (MAPT) is a ~76 kDa protein. Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Mouse MAPT/Tau/PHF-tau Antibody (TAU-5)
Flow cytometry

Flow-cytometry using anti-human Tau antibody. 5637 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Tau monoclonal antibody (Catalog MY086013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Mouse MAPT/Tau/PHF-tau Recombinant Antibody (TAU-5).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse MAPT/Tau/PHF-tau Recombinant Antibody (TAU-5) (MY086013)

Overview

Images

References

Datasheet

SDS

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