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Anti-Mouse MLKL Polyclonal Antibody (MV568014)

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Overview
Catalog No.MV568014
Description
Anti-Mouse MLKL Polyclonal Antibody (MV568014) is a rabbit polyclonal antibody detecting Mixed lineage kinase domain-like protein; Mlkl in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant mouse MLKL (Asp180-Val472).
Target Mixed lineage kinase domain-like protein; Mlkl
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q9D2Y4
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Mixed lineage kinase domain-like protein (MLKL) is a ~54 kDa protein. Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Does not have protein kinase activity. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function.

1. Wu, J. et al. (2013) Cell research 23, 994-1006. PMID: 23835476
2. Murphy, JM. et al. (2013) Immunity 39, 443-53. PMID: 24012422
3. Kaiser, WJ. et al. (2013) The Journal of biological chemistry 288, 31268-79. PMID: 24019532
4. Nogusa, S. et al. (2016) Cell host & microbe 20, 13-24. PMID: 27321907
5. Zhang, T. et al. (2020) Cell 180, 1115-1129.e13. PMID: 32200799
6. Jiao, H. et al. (2020) Nature 580, 391-395. PMID: 32296175
Note For research use only
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Datasheet
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Formula
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Anti-Mouse MLKL Polyclonal Antibody

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