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Anti-Mouse NUCB2 Polyclonal Antibody (MX912014)

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Anti-Mouse NUCB2 Polyclonal Antibody
Anti-Mouse NUCB2 Polyclonal Antibody
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Overview
Catalog No.MX912014
Description
Anti-Mouse NUCB2 Polyclonal Antibody (MX912014) is a rabbit polyclonal antibody detecting Nucb2 in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse NUCB2 (Gly51-Leu229).
Target Nucleobindin-2, DNA-binding protein NEFA, Prepronesfatin, Nesfatin-1, Nucb2, Nefa
Purification Purified by antigen affinity column.
Accession P81117
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Nucleobindin-2 (NUCB2) is a ~50 kDa protein. Calcium-binding protein which may have a role in calcium homeostasis. Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein (G-protein) alpha subunit GNAI3.

1. Taniguchi, N. et al. (2000) The Journal of biological chemistry 275, 31674-81. PMID: 10915798
Note For research use only.
Images
  • Anti-Mouse NUCB2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-NUCB2 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse spleen lysate
    Lane 2: Mouse testis lysate
    Lane 3: Rat testis lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Mouse NUCB2 Polyclonal Antibody

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