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Anti-Mouse Osteocalcin/BGLAP/OC Polyclonal Antibody (MY360014)

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Overview
Catalog No.MY360014
Description
Anti-Mouse Osteocalcin/BGLAP/OC Polyclonal Antibody (MY360014) is a rabbit polyclonal antibody detecting Osteocalcin in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse Osteocalcin/BGLAP/OC (Lys24-Ile95).
Target Osteocalcin, OG1, Bone Gla protein, BGP, Gamma-carboxyglutamic acid-containing protein, Bglap, Ocn
Purification Purified by antigen affinity column.
Accession P86546
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Osteocalcin (BGLAP) is a ~10 kDa protein. The carboxylated form is one of the main organic components of the bone matrix, which constitutes 1-2% of the total bone protein. Acts as a negative regulator of bone formation and is required to limit bone formation without impairing bone resorption or mineralization. Binds strongly to apatite and calcium upon gamma-carboxylation; this modification is essential for bone metabolism.

1. Ducy, P. et al. (1996) Nature 382, 448-52. PMID: 8684484
2. Lee, NK. et al. (2007) Cell 130, 456-69. PMID: 17693256
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Mouse Osteocalcin/BGLAP/OC Polyclonal Antibody

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