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Anti-Mouse PRDX6 Polyclonal Antibody (MB999014)

Anti-Mouse PRDX6 Polyclonal Antibody
Anti-Mouse PRDX6 Polyclonal Antibody
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Overview
Catalog No.MB999014
Description
Anti-Mouse PRDX6 Polyclonal Antibody (MB999014) is a rabbit polyclonal antibody detecting Prdx6. Suitable for Rat and Mouse.
Highlights
  • Multi-Species — Cross-reactive for translational research.
Species reactivityMouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse PRDX6 (Met1-Pro224).
Target 1-Cys PRX, 1-Cys peroxiredoxin, Acidic calcium-independent phospholipase A2, Antioxidant protein 2, Aop2, EC:1.11.1.27, EC:2.3.1.23, EC:3.1.1.4, Glutathione-dependent peroxiredoxin, LPC acyltransferase 5, LPCAT-5, Ltw4, Lyso-PC acyltransferase 5, Lysophosphatidylcholine acyltransferase 5, NSGPx, Non-selenium glutathione peroxidase, Peroxiredoxin-6, Prdx5, Prdx6, aiPLA2
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O08709
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Peroxiredoxin-6 (PRDX6) is a ~24 kDa protein. Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. Has phospholipase activity. Can either reduce the oxidized sn-2 fatty acyl group of phospholipids (peroxidase activity) or hydrolyze the sn-2 ester bond of phospholipids (phospholipase activity). These activities are dependent on binding to phospholipids at acidic pH and to oxidized phospholipds at cytosolic pH.

1. Fisher, AB. et al. (2016) Journal of lipid research 57, 587-96. PMID: 26830860
Note For research use only
Images
  • Anti-Mouse PRDX6 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PRDX6 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: K562 cell lysate
    Lane 2: Mouse brain lysate
    Lane 3: Mouse liver lysate
    Lane 4: Mouse spleen lysate
    Lane 5: Rat brain lysate
    Lane 6: Rat liver lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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