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Anti-Human/Mouse/Rat CD47 Recombinant Antibody (MIAP410) (HC359033)

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Overview
Catalog No.HC359033
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, FCM, Functional assay, Research in vivo
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG1, kappa
Clone IDMIAP410
Target Antigenic surface determinant protein OA3, CD47, IAP, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, Protein MER6
Endotoxin level < 10 EU/mg
Purity >95% as determined by SDS-PAGE.
Associated products pH 7.4 Dilution Buffer
Purification Protein A/G purified from cell culture supernatant.
Accession Q08722, Q08722, P97829
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Leukocyte surface antigen CD47 is a ~35 kDa protein. Adhesive protein that mediates cell-to-cell interactions. Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins. Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation. Plays a role in modulating pulmonary endothelin EDN1 signaling. Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure.

1. Latour, S. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 167, 2547-54. PMID: 11509594
2. Piccio, L. et al. (2005) Blood 105, 2421-7. PMID: 15383453
3. Isenberg, JS. et al. (2009) The Journal of biological chemistry 284, 1116-25. PMID: 19004835
4. Lindberg, FP. et al. (1993) The Journal of cell biology 123, 485-96. PMID: 7691831
5. Gao, AG. et al. (1996) The Journal of biological chemistry 271, 21-4. PMID: 8550562
6. Rogers, NM. et al. (2017) Cardiovascular research 113, 15-29. PMID: 27742621
7. Ghimire, K. et al. (2020) Cells 9. PMID: 32679764
Note For research use only
Isotype Control Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)
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Datasheet
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Formula
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Anti-Human/Mouse/Rat CD47 Recombinant Antibody (MIAP410)

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