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Anti-Mouse SFRP4 Polyclonal Antibody (MV362014)

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Overview
Catalog No.MV362014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse SFRP4 (Glu25-Asn116).
Target Secreted frizzled-related sequence protein 4 (FRP-4) (sFRP-4)
Purification Purified by antigen affinity column.
Accession Q9Z1N6
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Secreted frizzled-related sequence protein 4 (SFRP4) is a ~40 kDa protein. Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 plays a role in bone morphogenesis. May also act as a regulator of adult uterine morphology and function. May also increase apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling.

1. Kiper, POS. et al. (2016) The New England journal of medicine 374, 2553-2562. PMID: 27355534
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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C₂ (Working Conc.)
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Anti-Mouse SFRP4 Polyclonal Antibody

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