Anti-Mouse SPHK2 Polyclonal Antibody (MP741014)

Name: Anti-Mouse SPHK2 Polyclonal Antibody
Brand: abinScience
SKU: MP741014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

MP741014

Description

Anti-Mouse SPHK2 Polyclonal Antibody (MP741014) is a rabbit polyclonal antibody detecting SPHK2 in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse SPHK2 (Ile15-Gly276).

Target

SK2, Sphingosine kinase 2, SPHK2, SK 2, SPK 2

Purification

Purified by antigen affinity column.

Accession

Q9JIA7

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Sphingosine kinase 2 (SPHK2) is a ~65 kDa protein. Catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides. In contrast to prosurvival SPHK1, has a positive effect on intracellular ceramide levels, inhibits cells growth and enhances apoptosis. In mitochondria, is important for cytochrome-c oxidase assembly and mitochondrial respiration.

1. Maceyka, M. et al. (2005) The Journal of biological chemistry 280, 37118-29. PMID: 16118219

2. Olivera, A. et al. (2007) Immunity 26, 287-97. PMID: 17346996

3. Hisano, Y. et al. (2011) The Journal of biological chemistry 286, 1758-66. PMID: 21084291

4. Liu, H. et al. (2000) The Journal of biological chemistry 275, 19513-20. PMID: 10751414

5. Strub, GM. et al. (2011) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 25, 600-12. PMID: 20959514

6. Sivasubramanian, M. et al. (2015) Neuroscience 290, 636-48. PMID: 25637806

7. Ravichandran, S. et al. (2019) Biochimica et biophysica acta. Molecular basis of disease 1865, 570-576. PMID: 30593892

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-SPHK2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HCT116 cell lysate
Lane 2: Raji cell lysate
Lane 3: LO2 cell lysate

References

Datasheet

Anti-Mouse SPHK2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse SPHK2 Polyclonal Antibody (MP741014)

Overview

Images

References

Datasheet

SDS

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