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Anti-Mouse ST6GALNAC2/SIAT7-B Polyclonal Antibody (MP041014)

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Anti-Mouse ST6GALNAC2/SIAT7-B Polyclonal Antibody
Anti-Mouse ST6GALNAC2/SIAT7-B Polyclonal Antibody
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Overview
Catalog No.MP041014
Species reactivityMouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mouse ST6GALNAC2/SIAT7-B (Thr97-Arg373).
Target Alpha-N-acetylgalactosaminide alpha-2, 6-sialyltransferase 2 (EC 2.4.3.3) (Gal-beta-1, 3-GalNAc alpha-2, 6-sialyltransferase) (GalNAc alpha-2, 6-sialyltransferase II) (ST6GalNAc II) (ST6GalNAcII) (Sialyltransferase 7B) (SIAT7-B)
Purification Purified by antigen affinity column.
Accession P70277
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2 (ST6GALNAC2/SIAT7-B) is a ~42 kDa protein. Catalyzes the transfer of N-acetylneuraminyl groups onto glycan chains in glycoproteins. Conjugates sialic acid with an alpha-2-6 linkage to N-acetylgalactosamine (GalNAc) glycan chains linked to serine or threonine in glycoproteins. Sialylates alphaGalNAc- and Galbeta1->3GalNAc-O-Ser/Thr epitopes also known as Tn and T antigens.

1. Kurosawa, N. et al. (1996) The Journal of biological chemistry 271, 15109-16. PMID: 8662927
Note For research use only.
Images
  • Anti-Mouse ST6GALNAC2/SIAT7-B Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ST6GALNAC2 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse liver lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Mouse ST6GALNAC2/SIAT7-B Polyclonal Antibody

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