Anti-Mouse TCR β Recombinant Antibody (H57-597) (MP468013)

Name: Anti-Mouse TCR β Recombinant Antibody (H57-597)
Brand: abinScience
SKU: MP468013
Price: 191 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

MP468013

Description

Anti-Mouse TCR β Recombinant Antibody (H57-597) (MP468013) is a armenian hamster monoclonal antibody detecting TCRβ in ELISA. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.

Species reactivity

Mouse

Applications

ELISA

Host species

Armenian Hamster

Clonality

Monoclonal

Isotype

IgG

Clone ID

H57-597

Target

TCR β

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P01852

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

T cell receptor beta variable 12-3 (TCRβ) is a ~12 kDa protein. V region of the variable domain of T cell receptor (TR) beta chain that participates in the antigen recognition. Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens. Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome.

1. Lefranc, MP. (2014) Frontiers in immunology 5, 22. PMID: 24600447

2. Rossjohn, J. et al. (2015) Annual review of immunology 33, 169-200. PMID: 25493333

3. Brownlie, RJ. et al. (2013) Nature reviews. Immunology 13, 257-69. PMID: 23524462

4. Nikolich-Zugich, J. et al. (2004) Nature reviews. Immunology 4, 123-32. PMID: 15040585

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Mouse TCR β Antibody (H57-597)

References

Datasheet

Anti-Mouse TCR β Recombinant Antibody (H57-597).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse TCR β Recombinant Antibody (H57-597) (MP468013)

Overview

Images

References

Datasheet

SDS