Anti-Mouse TP53/p53 Polyclonal Antibody (MY039014)

Name: Anti-Mouse TP53/p53 Polyclonal Antibody
Brand: abinScience
SKU: MY039014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

MY039014

Description

Anti-Mouse TP53/p53 Polyclonal Antibody (MY039014) is a rabbit polyclonal antibody detecting TP53 in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse TP53/p53 (Met1-Asp390).

Target

Antigen NY-CO-13, Cellular tumor antigen p53, P53, Tumor suppressor p53, TP53, Phosphoprotein p53

Purification

Purified by antigen affinity column.

Accession

P02340

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cellular tumor antigen p53 (TP53) is a ~43 kDa protein. Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence. Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Negatively regulates cell division by controlling expression of a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression.

1. Allton, K. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 11612-6. PMID: 19556538

2. Huarte, M. et al. (2010) Cell 142, 409-19. PMID: 20673990

3. Vaseva, AV. et al. (2012) Cell 149, 1536-48. PMID: 22726440

4. Miki, T. et al. (2013) Nature communications 4, 2444. PMID: 24051492

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-TP53 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HT-29 cell lysate
Lane 2: 293T cell lysate
Lane 3: Raji cell lysate