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Anti-MPP5 Polyclonal Antibody (HV348014)

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Overview
Catalog No.HV348014
Description
Anti-MPP5 Polyclonal Antibody (HV348014) is a rabbit polyclonal antibody detecting MPP5 in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human MPP5 (Asn455-Arg675).
Target PALS1, MAGUK p55 subfamily member 5, MPP5
Purification Purified by antigen affinity column.
Accession Q8N3R9
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Protein PALS1 (MPP5) is a ~77 kDa protein. Plays a role in tight junction biogenesis and in the establishment of cell polarity in epithelial cells. Also involved in adherens junction biogenesis by ensuring correct localization of the exocyst complex protein EXOC4/SEC8 which allows trafficking of adherens junction structural component CDH1 to the cell surface. Plays a role through its interaction with CDH5 in vascular lumen formation and endothelial membrane polarity. Required during embryonic and postnatal retinal development. Required for the maintenance of cerebellar progenitor cells in an undifferentiated proliferative state, preventing premature differentiation, and is required for cerebellar histogenesis, fissure formation, cerebellar layer organization and cortical development.

1. Wells, CD. et al. (2006) Cell 125, 535-48. PMID: 16678097
2. Li, Y. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 17444-9. PMID: 25385611
3. Brinkmann, BF. et al. (2016) Molecular biology of the cell 27, 2811-21. PMID: 27466317
4. Carvalho, G. et al. (2011) PloS one 6, e18159. PMID: 21479189
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-MPP5 Polyclonal Antibody

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