Anti-MRV-3 Sigma1/S1 Polyclonal Antibody (VK094044)

Name: Anti-MRV-3 Sigma1/S1 Polyclonal Antibody
Brand: abinScience
SKU: VK094044
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD): --

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

VK094044

Description

Anti-MRV-3 Sigma1/S1 Polyclonal Antibody (VK094044) is a rabbit polyclonal antibody detecting MRV-3 Sigma1/S1 in ELISA, IHC, WB. Suitable for Bacillus phage SF6 and Reovirus type 3.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Mammalian orthoreovirus 1 (MRV1)

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant MRV-3 Sigma1/S1 (Val170-Thr455).

Target

sigma-1 protein, S1

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Accession

P03528, P21270

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Outer capsid protein sigma-1 (Sigma1/S1) is a ~49 kDa protein. Fiber-like molecule of about 30 nm that attaches the virion to the host cell membrane by binding with initial low affinity to sialic acid containing proteins, followed by high-affinity bindingto the entry receptor F11R/JAM-A. The interaction of sigma-1 with F11R is required for NF-kB activation and apoptosis. Binding to both sialic acid and F11R is required to induce maximal levels of apoptosis.

1. Barton, ES. et al. (2001) Cell 104, 441-51. PMID: 11239401

2. Kirchner, E. et al. (2008) PLoS pathogens 4, e1000235. PMID: 19079583

3. Reiter, DM. et al. (2011) PLoS pathogens 7, e1002166. PMID: 21829363

Note

For research use only

Images

References

Datasheet

Anti-MRV-3 Sigma1/S1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-MRV-3 Sigma1/S1 Polyclonal Antibody (VK094044)

Overview

Images

References

Datasheet

SDS