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Anti-MuV HN/Hemagglutinin-neuraminidase Polyclonal Antibody (VK637014)

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Overview
Catalog No.VK637014
Description
Anti-MuV HN/Hemagglutinin-neuraminidase Polyclonal Antibody (VK637014) is a rabbit polyclonal antibody detecting hemagglutinin-neuraminidase protein in ELISA, IHC, WB. Suitable for Mumps virus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityMumps virus (MuV)
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant MuV HN/Hemagglutinin-neuraminidase (Ile132-Ala322).
Target hemagglutinin-neuraminidase protein, HN
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P11235, P10866, P19762
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Hemagglutinin-neuraminidase (HN) is a ~64 kDa protein. Attaches the virus to alpha-2,3-linked sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion. Binds to the glycan motifs sialyl Lewis (SLe) and GM2 ganglioside (GM2-glycan).

Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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