Anti-Mycobacterium tuberculosis icl1/Isocitrate lyase 1 Recombinant Antibody (SAA2176) (JN930013)

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Overview

Catalog No.

JN930013

Description

Anti-Mycobacterium tuberculosis icl1/Isocitrate lyase 1 Antibody (SAA2176) [SAA2176] (JN930013) is a human monoclonal antibody detecting Isocitrate lyase 1 in ELISA.

Species reactivity

Mycobacterium tuberculosis (strain ATCC 35801 / TMC 107 / Erdman), Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh), Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Applications

ELISA

Host species

Human

Isotype

IgG1

Clone ID

SAA2176

Clonality

Monoclonal

Target

Isocitrate lyase 1, ICL1, 4.1.3.1, Isocitrase, Isocitratase, Methylisocitrate lyase, MICA, 4.1.3.30, icl1, ERDMAN_0512, Q643_00485, MT0483, Rv0467, MTV038.11

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

H8EVV4, P0A5H3, P9WKK6, P9WKK7

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Isocitrate lyase 1 (icl1) is a ~47 kDa protein. Involved in the persistence and virulence of M.tuberculosis. Catalyzes the reversible formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle during growth on fatty acid substrates. It also catalyzes the formation of pyruvate and succinate from 2-methylisocitrate, a key step in the methylcitrate cycle (propionate degradation route).

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Mycobacterium tuberculosis icl1/Isocitrate lyase 1 Antibody (SAA2176)

References

Datasheet

Anti-Mycobacterium tuberculosis icl1/Isocitrate lyase 1 Recombinant Antibody (SAA2176).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mycobacterium tuberculosis icl1/Isocitrate lyase 1 Recombinant Antibody (SAA2176) (JN930013)

Overview

Images

References

Datasheet

SDS

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