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Anti-Mycoplasma genitalium GAPDH/gapA Polyclonal Antibody (JN130044)

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Overview
Catalog No.JN130044
Description
Anti-Mycoplasma genitalium GAPDH/gapA Polyclonal Antibody (JN130044) is a rabbit polyclonal antibody detecting GAPDH. Suitable for Mycoplasma genitalium.
Species reactivityMycoplasma genitalium
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mycoplasma genitalium GAPDH/gapA (Met1-Leu337).
Target EC:1.2.1.12, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, NAD-dependent glyceraldehyde-3-phosphate dehydrogenase, gap, gapA
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P47543
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH/gapA) is a ~37 kDa protein. Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.

Note For research use only
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Anti-Mycoplasma genitalium GAPDH/gapA Polyclonal Antibody

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