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Anti-MYL12B Polyclonal Antibody (HT452014)

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Overview
Catalog No.HT452014
Description
Anti-MYL12B Polyclonal Antibody (HT452014) is a rabbit polyclonal antibody detecting MYL12B in ELISA, IHC, WB. Suitable for Bovine, Chicken, Human, Mouse, and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human MYL12B (Lys5-Asp172).
Target Myosin regulatory light chain MRLC2, MLC20, MYL12B, Myosin regulatory light chain 12B, Myosin regulatory light chain 20 kDa, MYLC2B, Myosin regulatory light chain 2-B, smooth muscle isoform, SHUJUN-1, MLC-2, MRLC2, MLC-2A
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O14950
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Myosin regulatory light chain 12B (MYL12B) is a ~19 kDa protein. Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion.

Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-MYL12B Polyclonal Antibody

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