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Anti-MYLK Polyclonal Antibody (HS792014)

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Overview
Catalog No.HS792014
Description
Anti-MYLK Polyclonal Antibody (HS792014) is a rabbit polyclonal antibody detecting MYLK in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Ovis aries.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human MYLK (Thr1748-Glu1914).
Target MLCK1, smMLCK, MYLK1, KRP, Kinase-related protein, MLCK, MYLK, Myosin light chain kinase, smooth muscle, Telokin
Purification Purified by antigen affinity column.
Accession Q15746
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Myosin light chain kinase, smooth muscle (MYLK) is a ~210 kDa protein. Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma.

Note For research use only.
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Formula
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Formula
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-MYLK Polyclonal Antibody

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