Anti-NARS1 Polyclonal Antibody (HT196014)

Name: Anti-NARS1 Polyclonal Antibody
Brand: abinScience
SKU: HT196014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HT196014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NARS1 (Met1-Arg330).

Target

Asparagine--tRNA ligase, cytoplasmic, NARS, NRS, Asparaginyl-tRNA synthetase, NARS1, Asparaginyl-tRNA synthetase 1, AsnRS

Purification

Purified by antigen affinity column.

Accession

O43776

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Asparagine--tRNA ligase, cytoplasmic (NARS1) is a ~62 kDa protein. Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction: asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn). In addition to its essential role in protein synthesis, acts as a signaling molecule that induced migration of CCR3-expressing cells. Has an essential role in the development of the cerebral cortex, being required for proper proliferation of radial glial cells.

1. Manole, A. et al. (2020) American journal of human genetics 107, 311-324. PMID: 32738225

2. Wang, L. et al. (2020) Nature communications 11, 4038. PMID: 32788587

3. Beaulande, M. et al. (1998) Nucleic acids research 26, 521-4. PMID: 9421509

4. Howard, OM. et al. (2002) The Journal of experimental medicine 196, 781-91. PMID: 12235211

5. Park, JS. et al. (2018) International journal of biological macromolecules 120, 835-845. PMID: 30171954

Note

For research use only.

Images

Western blot

Lane 1: HepG2 cell lysate
Lane 2: K562 cell lysate
Lane 3: Hela cell lysate
Lane 4: RAW264.7 cell lysate
Lane 5: 293T cell lysate
Lane 6: LO2 cell lysate
Lane 7: Jurkat cell lysate

References

Datasheet

Anti-NARS1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-NARS1 Polyclonal Antibody (HT196014)

Overview

Images

References

Datasheet

SDS

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