Anti-NCF1 Polyclonal Antibody (HB843014)

Name: Anti-NCF1 Polyclonal Antibody
Brand: abinScience
SKU: HB843014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HB843014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NCF1 (Met1-Val390).

Target

SH3PXD1A, NCF1, p47-phox, 47 kDa autosomal chronic granulomatous disease protein, Nox-organizing protein 2, NOXO2, SH3 and PX domain-containing protein 1A, NCF-1, 47 kDa neutrophil oxidase factor, NCF-47K, Nox organizer 2, Neutrophil NADPH oxidase factor 1, Neutrophil cytosol factor 1

Purification

Purified by antigen affinity column.

Accession

P14598

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Neutrophil cytosol factor 1 (NCF1) is a ~44 kDa protein. Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction. Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane. This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox.

1. Lomax, KJ. et al. (1989) Science (New York, N.Y.) 245, 409-12. PMID: 2547247

2. Volpp, BD. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 7195-9. PMID: 2550933

3. Liu, X. et al. (2024) Nature 627, 189-195. PMID: 38355798

4. Groemping, Y. et al. (2003) Cell 113, 343-55. PMID: 12732142

5. Kilpatrick, LE. et al. (2010) Journal of leukocyte biology 87, 153-64. PMID: 19801500

Note

For research use only.

Images

References

Datasheet

Anti-NCF1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-NCF1 Polyclonal Antibody (HB843014)

Overview

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References

Datasheet

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