Anti-NCOA2 Polyclonal Antibody (HA816014)

Name: Anti-NCOA2 Polyclonal Antibody
Brand: abinScience
SKU: HA816014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HA816014

Description

Anti-NCOA2 Polyclonal Antibody (HA816014) is a rabbit polyclonal antibody detecting Nuclear receptor coactivator 2; NCoA-2; Class E basic helix-loop-helix protein 75; bHLHe75; Transcriptional intermediary factor 2; hTIF2; NCOA2; BHLHE75; SRC2; TIF2 in ELISA, IHC, WB. Suitable for Human, Rat, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human NCOA2 (Val109-Cys380).

Target

Nuclear receptor coactivator 2; NCoA-2; Class E basic helix-loop-helix protein 75; bHLHe75; Transcriptional intermediary factor 2; hTIF2; NCOA2; BHLHE75; SRC2; TIF2

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q15596, Q9WUI9, Q61026

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Nuclear receptor coactivator 2 (NCOA2) is a ~159 kDa protein. Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues. Critical regulator of glucose metabolism regulation, acts as a RORA coactivator to specifically modulate G6PC1 expression. Involved in the positive regulation of the transcriptional activity of the glucocorticoid receptor NR3C1 by sumoylation enhancer RWDD3.

1. Druker, J. et al. (2013) Molecular and cellular biology 33, 2116-27. PMID: 23508108

2. Voegel, JJ. et al. (1996) The EMBO journal 15, 3667-75. PMID: 8670870

3. Voegel, JJ. et al. (1998) The EMBO journal 17, 507-19. PMID: 9430642

4. Musille, PM. et al. (2012) Nature structural & molecular biology 19, 532-S2. PMID: 22504882

5. Musille, PM. et al. (2016) The Journal of biological chemistry 291, 1411-26. PMID: 26553876

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-NCOA2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: Jurkat cell lysate

References

Datasheet

Anti-NCOA2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-NCOA2 Polyclonal Antibody (HA816014)

Overview

Images

References

Datasheet

SDS

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